This book constitutes the refereed proceedings of the 16th International Symposium on Pervasive Systems, Algorithms and Networks, I-SPAN 2019, held in Naples, Italy, in September 2019. The 32 full papers and 8 short papers were carefully reviewed and selected from 89 submissions. The papers focus on all aspects of: big data analytics & machine learning; cyber security; cloud fog & edge computing; communication solutions; high performance computing and applications; consumer cyber security; and vehicular technology.