This book constitutes the thoroughly refereed post-conference proceedings of the 13th International Meeting on Computational Intelligence Methods  for Bioinformatics and Biostatistics, CIBB 2016, held in Stirling, UK, in September 2016. The 19 revised full papers and 6 keynotes abstracts presented were carefully reviewed and selected from 61 submissions. The papers  deal with the application of computational intelligence to open problems in bioinformatics, biostatistics, systems and synthetic biology, medicalinformatics, computational approaches to life sciences in general.