The conference proceeding LNCS 11346 constitutes the refereed proceedings of the 12th International Conference on Combinatorial Optimization and Applications, COCOA 2018, held in Atlanta, GA, USA, in December 2018. The 50 full papers presented were carefully reviewed and selected from 106 submissions. The papers cover most aspects of t graph algorithms, routing and network design problems, scheduling algorithms, network optimization, combinatorial algorithms, approximation algorithms, paths and connectivity problems and much more. .